Efficient generation of recombinant adenoviral vectors by Cre-lox recombination in vitro
K Aoki, C Barker, X Danthinne, MJ Imperiale… - Molecular …, 1999 - Springer
K Aoki, C Barker, X Danthinne, MJ Imperiale, GJ Nabel
Molecular Medicine, 1999•SpringerBackground Although recombinant adenovirus vectors are attractive for use in gene
expression studies and therapeutic applications, the construction of these vectors remains
relatively time-consuming. We report here a strategy that simplifies the production of
adenoviruses using the Cre-loxP system. Materials and Methods Full-length recombinant
adenovirus DNA was generated in vitro by Cre-mediated recombination between loxP sites
in a linearized shuttle plasmid containing a transgene and adenovirus genomic DNA …
expression studies and therapeutic applications, the construction of these vectors remains
relatively time-consuming. We report here a strategy that simplifies the production of
adenoviruses using the Cre-loxP system. Materials and Methods Full-length recombinant
adenovirus DNA was generated in vitro by Cre-mediated recombination between loxP sites
in a linearized shuttle plasmid containing a transgene and adenovirus genomic DNA …
Background
Although recombinant adenovirus vectors are attractive for use in gene expression studies and therapeutic applications, the construction of these vectors remains relatively time-consuming. We report here a strategy that simplifies the production of adenoviruses using the Cre-loxP system.
Materials and Methods
Full-length recombinant adenovirus DNA was generated in vitro by Cre-mediated recombination between loxP sites in a linearized shuttle plasmid containing a transgene and adenovirus genomic DNA.
Results
After transfection of Cre-treated DNA into 293 cells, replication-defective viral vectors were rapidly obtained without detectable wild-type virus.
Conclusion
This system facilitates the development of recombinant adenoviral vectors for basic and clinical research.
Springer