A novel method based on flow cytometry (FCM), which can count the number of detected
cells, has been developed for the evaluation of cellular proliferation and cytotoxicity in vitro.
It provides a tool that directly counts cell number without being influenced by the metabolic
state of the cells, discriminates target cells from effector cells in cell-mediated cytotoxicity
assay, and with less treatment step and free radioactivity. In this paper, we have prepared
the PG cells (a highly metastatic human lung cancer cell line) and peripheral blood …