[HTML][HTML] The plant lectin FRIL supports prolonged in vitro maintenance of quiescent human cord blood CD34+ CD38−/low/SCID repopulating stem cells
O Kollet, JG Moore, R Aviram, H Ben-Hur, BL Liu… - Experimental …, 2000 - Elsevier
OBJECTIVE: Ex vivo maintenance of human stem cells is crucial for many clinical
applications. Current culture methods rely on optimized combinations of cytokines. Although
these conditions provide some level of stem cell support, they primarily induce proliferation
and differentiation, resulting in reduced repopulation capacity. MATERIALS AND
METHODS: The recently identified legume lectin FRIL has been shown to preserve human
cord blood progenitors up to a month in suspension culture without medium changes. To test …
applications. Current culture methods rely on optimized combinations of cytokines. Although
these conditions provide some level of stem cell support, they primarily induce proliferation
and differentiation, resulting in reduced repopulation capacity. MATERIALS AND
METHODS: The recently identified legume lectin FRIL has been shown to preserve human
cord blood progenitors up to a month in suspension culture without medium changes. To test …