[HTML][HTML] Regulated expression, processing, and secretion of dog mast cell dipeptidyl peptidase I
PJ Wolters, WW Raymond, JL Blount… - Journal of Biological …, 1998 - ASBMB
Dipeptidyl peptidase I (DPPI) is a cysteine protease found predominantly in myelomonocytic
cells, cytotoxic T-cells, and mast cells. Recent studies identify an intracellular role for mast
cell-DPPI (MC-DPPI) by activating prochymase and protryptase to their mature forms. To
better define MC-DPPI and to explore the possibility of extracellular roles, we purified MC-
DPPI from mastocytoma cells. We found the dog C2 mastocytoma cell line to be the richest
source yet described for DPPI, purifying up to 200 μg of enzyme per g of cells. Dog MC-DPPI …
cells, cytotoxic T-cells, and mast cells. Recent studies identify an intracellular role for mast
cell-DPPI (MC-DPPI) by activating prochymase and protryptase to their mature forms. To
better define MC-DPPI and to explore the possibility of extracellular roles, we purified MC-
DPPI from mastocytoma cells. We found the dog C2 mastocytoma cell line to be the richest
source yet described for DPPI, purifying up to 200 μg of enzyme per g of cells. Dog MC-DPPI …
