Selection of highly transfectable variant from mouse mastocytoma P815
A Van Pel, E De Plaen, T Boon - Somatic cell and molecular genetics, 1985 - Springer
A Van Pel, E De Plaen, T Boon
Somatic cell and molecular genetics, 1985•SpringerA tk− cell line derived from mouse mastocytoma P815 was transfected with a plasmid
carrying a thymidine kinase gene. The tk+ cells were obtained at a frequency of 10− 6. From
some of these tk+ transfectants it was possible to select tk− cells with BrdU so that these
cells could be submitted again to transfection with the thymidine kinase gene. By repeating
cycles of transfection and tk+ selection followed by reverse selection with BrdU, it was
possible to obtain a stable variant having a 100-fold increase in transfection efficiency. This …
carrying a thymidine kinase gene. The tk+ cells were obtained at a frequency of 10− 6. From
some of these tk+ transfectants it was possible to select tk− cells with BrdU so that these
cells could be submitted again to transfection with the thymidine kinase gene. By repeating
cycles of transfection and tk+ selection followed by reverse selection with BrdU, it was
possible to obtain a stable variant having a 100-fold increase in transfection efficiency. This …
Abstract
A tk− cell line derived from mouse mastocytoma P815 was transfected with a plasmid carrying a thymidine kinase gene. The tk+ cells were obtained at a frequency of 10−6. From some of these tk+ transfectants it was possible to select tk− cells with BrdU so that these cells could be submitted again to transfection with the thymidine kinase gene. By repeating cycles of transfection and tk+ selection followed by reverse selection with BrdU, it was possible to obtain a stable variant having a 100-fold increase in transfection efficiency. This HTR (high transfection) variant shows a high efficiency of transfection (10−4) for the neomycin-resistance gene as well as for the thymidine kinase gene.
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