Direct demonstration of the infiltration of murine central nervous system by Pgp-1/CD44high CD45RBlow CD4+ T cells that induce experimental allergic …
R Zeine, T Owens - Journal of neuroimmunology, 1992 - Elsevier
In experimental allergic encephalomyelitis (EAE), autoimmune T cells infiltrate the central
nervous system (CNS) and initiate demyelinating pathology. We have used flow cytometry to
directly analyse the migration to the CNS of MBP-reactive CD4+ T cells labelled with a
lipophilic fluorescent dye (PKH2), in SJL/J mice with passively transferred EAE. Labelled
cells constituted about 45% of the CNS CD4+ population at the time of EAE onset. Almost all
(> 90%) of the PKH2-labelled CD4+ T cells from EAE CNS were blasts and were α/β T cell …
nervous system (CNS) and initiate demyelinating pathology. We have used flow cytometry to
directly analyse the migration to the CNS of MBP-reactive CD4+ T cells labelled with a
lipophilic fluorescent dye (PKH2), in SJL/J mice with passively transferred EAE. Labelled
cells constituted about 45% of the CNS CD4+ population at the time of EAE onset. Almost all
(> 90%) of the PKH2-labelled CD4+ T cells from EAE CNS were blasts and were α/β T cell …
