Primary bioassay of human tumor stem cells

AW Hamburger, SE Salmon - Science, 1977 - science.org
AW Hamburger, SE Salmon
Science, 1977science.org
A simple method has been developed to support human tumor stem cell colony growth in
soft agar. The technique appears suitable for culture of a variety of neoplasms of differing
histopathology. Tumor stem cell colonies arising from different types of cancer have differing
growth characteristics and colony morphology. This bioassay should be suitable for clinical
studies of effects of anticancer drugs or irradiation on human tumor stem cells.
A simple method has been developed to support human tumor stem cell colony growth in soft agar. The technique appears suitable for culture of a variety of neoplasms of differing histopathology. Tumor stem cell colonies arising from different types of cancer have differing growth characteristics and colony morphology. This bioassay should be suitable for clinical studies of effects of anticancer drugs or irradiation on human tumor stem cells.
AAAS