[HTML][HTML] Modified glutamine catabolism in macrophages of Ucp2 knock-out mice
T Nübel, Y Emre, D Rabier, B Chadefaux… - … et Biophysica Acta (BBA …, 2008 - Elsevier
T Nübel, Y Emre, D Rabier, B Chadefaux, D Ricquier, F Bouillaud
Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2008•ElsevierUncoupling protein 2 (UCP2) belongs to a family of transporters of the mitochondrial inner
membrane and is reported to uncouple respiration from ATP synthesis. Our observation that
the amino acid glutamine specifically induces UCP2 protein expression prompted us to
investigate metabolic consequences of a UCP2 knockdown (Ucp2-KO) when glutamine is
offered as a substrate. We found that Ucp2-KO macrophages incubated in the presence of
glutamine exhibit a lower ammonium release, a decreased respiratory rate, and an …
membrane and is reported to uncouple respiration from ATP synthesis. Our observation that
the amino acid glutamine specifically induces UCP2 protein expression prompted us to
investigate metabolic consequences of a UCP2 knockdown (Ucp2-KO) when glutamine is
offered as a substrate. We found that Ucp2-KO macrophages incubated in the presence of
glutamine exhibit a lower ammonium release, a decreased respiratory rate, and an …
Uncoupling protein 2 (UCP2) belongs to a family of transporters of the mitochondrial inner membrane and is reported to uncouple respiration from ATP synthesis. Our observation that the amino acid glutamine specifically induces UCP2 protein expression prompted us to investigate metabolic consequences of a UCP2 knockdown (Ucp2-KO) when glutamine is offered as a substrate. We found that Ucp2-KO macrophages incubated in the presence of glutamine exhibit a lower ammonium release, a decreased respiratory rate, and an intracellular accumulation of aspartate. Therefore, we conclude that UCP2 expression is required for efficient oxidation of glutamine in macrophages. This role of UCP2 in glutamine metabolism appears independent from the uncoupling activity of UCP2.
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