Alternative splicing of insulin-like growth factor I mRNA is developmentally regulated in the rat and mouse with preferential exon 2 usage in the mouse
W Lin, AM Oberbauer - Growth hormone & IGF research, 1998 - Elsevier
The synthesis of an IGF-I complementary RNA (cRNA) standard containing the primer
sequences for each of the four IGF-I mRNA alternative transcript forms (class 1Ea, 1Eb, 2Ea
and 2Eb) and a polyA+ tail allowed the determination of the absolute abundance of each
form in growing rats and mice by quantitative RT-PCR. In rat liver, class 1Ea mRNA was the
most abundant form representing 90% of the total IGF-I mRNA. Though the relative
proportion was maintained, the absolute abundance was maximal at 4 weeks of age and …
sequences for each of the four IGF-I mRNA alternative transcript forms (class 1Ea, 1Eb, 2Ea
and 2Eb) and a polyA+ tail allowed the determination of the absolute abundance of each
form in growing rats and mice by quantitative RT-PCR. In rat liver, class 1Ea mRNA was the
most abundant form representing 90% of the total IGF-I mRNA. Though the relative
proportion was maintained, the absolute abundance was maximal at 4 weeks of age and …