Characterization of prostaglandin production in tissue culture of rat renal medullary cells
MJ Dunn, RS Staley, M Harrison - Prostaglandins, 1976 - Elsevier
Renal medullary cells from the rat were used to establish a cell culture line. The morphologic
characteristics of these cells were similar to renal medullary interstitial cells. They produced
substantial amounts of PGE when provided with arachidonic acid or fetal calf serum. PGE
production was inhibited 80–90% by indomethacin or meclofenamate. PGE release by the
cells was sensitive to and stimulated by changing the culture media. Stable levels of PGE in
the media could be achieved if media changes were avoided during the experimental …
characteristics of these cells were similar to renal medullary interstitial cells. They produced
substantial amounts of PGE when provided with arachidonic acid or fetal calf serum. PGE
production was inhibited 80–90% by indomethacin or meclofenamate. PGE release by the
cells was sensitive to and stimulated by changing the culture media. Stable levels of PGE in
the media could be achieved if media changes were avoided during the experimental …
