[HTML][HTML] hScrib interacts with ZO-2 at the cell–cell junctions of epithelial cells
FEBS letters, 2005•Elsevier
In Drosophila, the tumor suppressor Scribble is localized at the septate junctions of epithelial
cells. Its mammalian homologue, hScrib, is a basolateral protein likely associated to proteins
of the cell–cell junctions. We report the direct interaction between hScrib and ZO-2, a
junction-associated protein. This interaction relies on two PDZ domains of hScrib and on the
C-terminal motif of ZO-2. Both proteins localise at cell–cell junctions of epithelial cells. A
point mutation in the LRR of hScrib delocalises the protein from the plasma membrane and …
cells. Its mammalian homologue, hScrib, is a basolateral protein likely associated to proteins
of the cell–cell junctions. We report the direct interaction between hScrib and ZO-2, a
junction-associated protein. This interaction relies on two PDZ domains of hScrib and on the
C-terminal motif of ZO-2. Both proteins localise at cell–cell junctions of epithelial cells. A
point mutation in the LRR of hScrib delocalises the protein from the plasma membrane and …
In Drosophila, the tumor suppressor Scribble is localized at the septate junctions of epithelial cells. Its mammalian homologue, hScrib, is a basolateral protein likely associated to proteins of the cell–cell junctions. We report the direct interaction between hScrib and ZO-2, a junction-associated protein. This interaction relies on two PDZ domains of hScrib and on the C-terminal motif of ZO-2. Both proteins localise at cell–cell junctions of epithelial cells. A point mutation in the LRR of hScrib delocalises the protein from the plasma membrane and abrogates the interaction with ZO-2 but not with βPIX. Tyrosine phosphorylation of hScrib does not impair the interaction with ZO-2. We show a direct link between two junctional proteins that are down-regulated during cancer progression.
Elsevier
