Synergistic inhibition of human immunodeficiency virus type 1 and type 2 replication in vitro by castanospermine and 3'-azido-3'-deoxythymidine
VA Johnson, BD Walker, MA Barlow… - Antimicrobial agents …, 1989 - Am Soc Microbiol
Antimicrobial agents and chemotherapy, 1989•Am Soc Microbiol
Castanospermine and 3'-azido-3'-deoxythymidine (zidovudine) were evaluated in
combination against human immunodeficiency virus (HIV) replication in vitro.
Castanospermine and 3'-azido-3'-deoxythymidine inhibited HIV type 1 synergistically in
acutely infected H9 cells. In addition, they synergistically inhibited both HIV type 1 and HIV
type 2 in peripheral blood mononuclear cells. There were no additional toxic effects of these
agents in combination. Drug interactions were evaluated by the median-effect principle and …
combination against human immunodeficiency virus (HIV) replication in vitro.
Castanospermine and 3'-azido-3'-deoxythymidine inhibited HIV type 1 synergistically in
acutely infected H9 cells. In addition, they synergistically inhibited both HIV type 1 and HIV
type 2 in peripheral blood mononuclear cells. There were no additional toxic effects of these
agents in combination. Drug interactions were evaluated by the median-effect principle and …
Castanospermine and 3'-azido-3'-deoxythymidine (zidovudine) were evaluated in combination against human immunodeficiency virus (HIV) replication in vitro. Castanospermine and 3'-azido-3'-deoxythymidine inhibited HIV type 1 synergistically in acutely infected H9 cells. In addition, they synergistically inhibited both HIV type 1 and HIV type 2 in peripheral blood mononuclear cells. There were no additional toxic effects of these agents in combination. Drug interactions were evaluated by the median-effect principle and the isobologram technique. Combinations of a glycosylation inhibitor, such as castanospermine, with 3'-azido-3'-deoxythymidine deserve consideration for HIV-related chemotherapeutic intervention.
American Society for Microbiology