Monitoring cytoskeletal dynamics in living neurons using fluorescence photoactivation
A Gauthier-Kemper, C Weissmann, HJ Reyher… - Methods in …, 2012 - Elsevier
Neurons exhibit high temporal and spatial dynamics of their cytoskeletal organization, which
is critical for the development and maintenance of axons and dendrites. Live cell imaging of
fluorescence labeled proteins provides a powerful approach to scrutinize the dynamics of
cytoskeletal components in living neuronal cells. Here, we describe a method to monitor and
quantitatively analyze the dissipation of populations of cytoskeletal proteins in neurites of
living cells using fluorescence photoactivation of fusion constructs with photoactivatable …
is critical for the development and maintenance of axons and dendrites. Live cell imaging of
fluorescence labeled proteins provides a powerful approach to scrutinize the dynamics of
cytoskeletal components in living neuronal cells. Here, we describe a method to monitor and
quantitatively analyze the dissipation of populations of cytoskeletal proteins in neurites of
living cells using fluorescence photoactivation of fusion constructs with photoactivatable …
