Targeting the reversibly oxidized protein tyrosine phosphatase superfamily
B Boivin, M Yang, NK Tonks - Science signaling, 2010 - science.org
B Boivin, M Yang, NK Tonks
Science signaling, 2010•science.orgControlled production of reactive oxygen species leads to reversible oxidation of protein
tyrosine phosphatases (PTPs) and has emerged as an important tier of regulation over
phosphorylation-dependent signal transduction. We present a modified cysteinyl-labeling
assay that detects reversible oxidation of members of each of the different PTP subclasses.
Here, we describe the methods for enriching reversibly oxidized PTPs from complex protein
extracts, illustrating the procedure in IMR90 fibroblasts.
tyrosine phosphatases (PTPs) and has emerged as an important tier of regulation over
phosphorylation-dependent signal transduction. We present a modified cysteinyl-labeling
assay that detects reversible oxidation of members of each of the different PTP subclasses.
Here, we describe the methods for enriching reversibly oxidized PTPs from complex protein
extracts, illustrating the procedure in IMR90 fibroblasts.
Controlled production of reactive oxygen species leads to reversible oxidation of protein tyrosine phosphatases (PTPs) and has emerged as an important tier of regulation over phosphorylation-dependent signal transduction. We present a modified cysteinyl-labeling assay that detects reversible oxidation of members of each of the different PTP subclasses. Here, we describe the methods for enriching reversibly oxidized PTPs from complex protein extracts, illustrating the procedure in IMR90 fibroblasts.
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