Assessment of human muscle glycogen synthesis and total glucose content by in vivo13C MRS
V Den Bergh, Tack, V Den Boogert… - European journal of …, 2000 - Wiley Online Library
V Den Bergh, Tack, V Den Boogert, Vervoort, Smits, Heerschap
European journal of clinical investigation, 2000•Wiley Online LibraryBackground Obesity is often accompanied by a decreased ability of insulin to stimulate
glucose uptake and glycogenesis in skeletal muscle. The aim of this study was to investigate
the rate of glycogen formation and of muscular glucose content in relation to insulin
sensitivity under euglycemic conditions. Materials and methods We applied a
hyperinsulinemic (430 pmol m− 2 min− 1) euglycemic clamp with infusion of 20% glucose
(30% enriched with 13C‐1‐glucose) to 8 subjects with a wide range of insulin sensitivities …
glucose uptake and glycogenesis in skeletal muscle. The aim of this study was to investigate
the rate of glycogen formation and of muscular glucose content in relation to insulin
sensitivity under euglycemic conditions. Materials and methods We applied a
hyperinsulinemic (430 pmol m− 2 min− 1) euglycemic clamp with infusion of 20% glucose
(30% enriched with 13C‐1‐glucose) to 8 subjects with a wide range of insulin sensitivities …
Background
Obesity is often accompanied by a decreased ability of insulin to stimulate glucose uptake and glycogenesis in skeletal muscle. The aim of this study was to investigate the rate of glycogen formation and of muscular glucose content in relation to insulin sensitivity under euglycemic conditions.
Materials and methods
We applied a hyperinsulinemic (430 pmol m−2 min−1) euglycemic clamp with infusion of 20% glucose (30% enriched with 13C‐1‐glucose) to 8 subjects with a wide range of insulin sensitivities. Glycogen and glucose levels were monitored simultaneously by in vivo13C MRS of the calf muscle on a clinical MR system at 1.5T field strength.
Results and conclusions
Glycogen synthesis rate showed a strong correlation with whole body glucose uptake during the clamp (r = 0.93, P < 0.01). With the use of 13C MRS, total muscular glucose content could be determined in vivo, and showed a positive, linear correlation with glycogen synthesis rate (r = 0.85, P < 0.01). 13C MRS provides important information regarding in vivo insulin action. Preliminary results indicate that the glycogen synthesis rate improves after treatment with troglitazone.
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