Transplantation of neonatal gut neural crest progenitors reconstructs ganglionic function in benzalkonium chloride-treated homogenic rat colon
WK Pan, BJ Zheng, Y Gao, H Qin, Y Liu - Journal of Surgical Research, 2011 - Elsevier
WK Pan, BJ Zheng, Y Gao, H Qin, Y Liu
Journal of Surgical Research, 2011•ElsevierBACKGROUND: To value the possibility and the future feasibility of the use of autograft cells
transplantation in disorders of the enteric neural system, we postulate that isolated neonatal
nongenetically modified neural crest progenitors could survive and differentiate into neurons
and glia in homogenic denervated rats and, therefore, restore partial intestinal function after
transplantation. METHODS: Neural crest progenitors were isolated from neonatal rats. After
passages, the cells were labeled with CM-DiI. The labeled cells were then delivered into the …
transplantation in disorders of the enteric neural system, we postulate that isolated neonatal
nongenetically modified neural crest progenitors could survive and differentiate into neurons
and glia in homogenic denervated rats and, therefore, restore partial intestinal function after
transplantation. METHODS: Neural crest progenitors were isolated from neonatal rats. After
passages, the cells were labeled with CM-DiI. The labeled cells were then delivered into the …
BACKGROUND
To value the possibility and the future feasibility of the use of autograft cells transplantation in disorders of the enteric neural system, we postulate that isolated neonatal nongenetically modified neural crest progenitors could survive and differentiate into neurons and glia in homogenic denervated rats and, therefore, restore partial intestinal function after transplantation.
METHODS
Neural crest progenitors were isolated from neonatal rats. After passages, the cells were labeled with CM-DiI. The labeled cells were then delivered into the muscular distal denervated colon of rats whose neural plexuses were eliminated using benzalkonium chloride. The treated colons of recipients were harvested at 1, 4, and 8 wk, and identified by immunofluorescent staining. The physiologic and functional improvements on treated colons were well examined after transplantation 8 wk.
RESULTS
Progenitors could generate neurospheres and differentiate into neurons and glia in vitro. After transplantation, red fluorescent cells were observed in the injected tissue for up to 8 wk, and they differentiated into neurons and glia in the host colon. Functional examinations indicated that symptoms and intestinal dysfunction of the denervated model were reversed.
CONCLUSIONS
We provide herein further evidence that autologous cell transplantation is a feasible therapy for enteric nervous system disorders.
Elsevier