Twenty-four hours after bacterial innoculation, when gross swelling was apparent in the thigh, one of the radiolabeled proteins (@ — 0.25 mg/kg labeled with 100— 150MCiof †œInfor each preparation) was injected intravenously via the tail vein. Following injection ofthe radiolabeled reagent, serial anterior whole-body scintigrams of ketamine anesthetized animals were acquired using a standard field of view scintillation camera equipped with a parallel-hole medium-energy colli mator interfaced to a dedicated computer system (Technicare 100, Technicare 450, Solon, Ohio). Images were recorded for a preset time of 10 mm/view with a window centered to include both the 173 and 247 keY photopeaks of †††In.