Physical properties of human α2-macroglobulin following reaction with methylamine and trypsin
SL Gonias, JA Reynolds, SV Pizzo - Biochimica et Biophysica Acta (BBA) …, 1982 - Elsevier
Circular dichroism spectroscopy, sedimentation velocity and ultraviolet difference
spectroscopy were used to compare α 2-macroglobulin, α 2-macroglobulin-trypsin complex
and α 2-macroglobulin-methylamine complex. The circular dichroic spectrum of native α 2-
macroglobulin is significantly changed in shape and magnitude following reaction with
either trypsin or methylamine. The spectra of α 2-macroglobulin-trypsin and α 2-
macroglobulin-methylamine are, however, indistinguishable. The ultraviolet difference …
spectroscopy were used to compare α 2-macroglobulin, α 2-macroglobulin-trypsin complex
and α 2-macroglobulin-methylamine complex. The circular dichroic spectrum of native α 2-
macroglobulin is significantly changed in shape and magnitude following reaction with
either trypsin or methylamine. The spectra of α 2-macroglobulin-trypsin and α 2-
macroglobulin-methylamine are, however, indistinguishable. The ultraviolet difference …