[HTML][HTML] Targeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling
Protein kinase C (PKC) family members transduce an abundance of diverse intracellular
signals. Here we address the role of spatial and temporal segregation in signal specificity by
measuring the activity of endogenous PKC at defined intracellular locations in real time in
live cells. We targeted a genetically encoded fluorescence resonance energy transfer-based
reporter for PKC activity, C kinase activity reporter (CKAR)(Violin, JD, Zhang, J., Tsien, RY,
and Newton, AC (2003) J. Cell Biol. 161, 899-909), to the plasma membrane, Golgi, cytosol …
signals. Here we address the role of spatial and temporal segregation in signal specificity by
measuring the activity of endogenous PKC at defined intracellular locations in real time in
live cells. We targeted a genetically encoded fluorescence resonance energy transfer-based
reporter for PKC activity, C kinase activity reporter (CKAR)(Violin, JD, Zhang, J., Tsien, RY,
and Newton, AC (2003) J. Cell Biol. 161, 899-909), to the plasma membrane, Golgi, cytosol …
