Viral microRNA genomics and target validation
JM Ziegelbauer - Current opinion in virology, 2014 - Elsevier
Highlights•Most viral microRNAs show little or no sequence similarity to other viral
microRNAs or cellular microRNAs.•There is no single quintessential target prediction
method, integration of various methods is key to filter and prioritize potential miRNA targets
for further analysis.•Secondary target validation assays are difficult to perform in a high-
throughput fashion.A subset of viruses express their own microRNAs (miRNAs) and one way
to understand the functions of these microRNAs is to identify the targets of these miRNAs …
microRNAs or cellular microRNAs.•There is no single quintessential target prediction
method, integration of various methods is key to filter and prioritize potential miRNA targets
for further analysis.•Secondary target validation assays are difficult to perform in a high-
throughput fashion.A subset of viruses express their own microRNAs (miRNAs) and one way
to understand the functions of these microRNAs is to identify the targets of these miRNAs …