Evaluation of differential toxicity of varying doses of bevacizumab on retinal ganglion cells, retinal pigment epithelial cells, and vascular endothelial growth factor …

VS Brar, RK Sharma, RK Murthy… - Journal of ocular …, 2009 - liebertpub.com
VS Brar, RK Sharma, RK Murthy, KV Chalam
Journal of ocular pharmacology and therapeutics, 2009liebertpub.com
Purpose: To evaluate in vitro the effects of bevacizumab, an anti-vascular endothelial growth
factor (VEGF) antibody, on retinal pigment epithelial cells (RPE) and retinal ganglion cells
(RGC), at doses that were inhibitory to VEGF-enriched choroidal endothelial cells (CEC).
Methods: Monkey CEC (RF6A), human RPE cells (ARPE-19), and rat RGC (RGC-5) were
exposed for 24 h to increasing doses of bevacizumab. Cell numbers were quantified with
WST-1 assay. Cell death was assessed using propidium iodide (PI) staining via flow …
Purpose: To evaluate in vitro the effects of bevacizumab, an anti-vascular endothelial growth factor (VEGF) antibody, on retinal pigment epithelial cells (RPE) and retinal ganglion cells (RGC), at doses that were inhibitory to VEGF-enriched choroidal endothelial cells (CEC).
Methods: Monkey CEC (RF6A), human RPE cells (ARPE-19), and rat RGC (RGC-5) were exposed for 24 h to increasing doses of bevacizumab. Cell numbers were quantified with WST-1 assay. Cell death was assessed using propidium iodide (PI) staining via flow cytometry and fluorescent microscopy.
Results: Bevacizumab was inhibitory to RF6A at 2.0 mg/mL (P < 0.005). No effect on cell viability was noted on ARPE-19 and RGC-5 cell lines at this particular dose of bevacizumab. These results were supported by fluorescent microscopy of PI-stained cells.
Conclusions: VEGF-stimulated proliferation of CEC was inhibited by bevacizumab. Bevacizumab was not cytotoxic to human RPE and rat RGC in vitro at a dose that is inhibitory to monkey CEC.
Mary Ann Liebert