[HTML][HTML] Mitochondria-targeted Ogg1 and aconitase-2 prevent oxidant-induced mitochondrial DNA damage in alveolar epithelial cells
SJ Kim, P Cheresh, D Williams, Y Cheng… - Journal of Biological …, 2014 - ASBMB
Mitochondria-targeted human 8-oxoguanine DNA glycosylase (mt-hOgg1) and aconitase-2
(Aco-2) each reduce oxidant-induced alveolar epithelial cell (AEC) apoptosis, but it is
unclear whether protection occurs by preventing AEC mitochondrial DNA (mtDNA) damage.
Using quantitative PCR-based measurements of mitochondrial and nuclear DNA damage,
mtDNA damage was preferentially noted in AEC after exposure to oxidative stress (eg
amosite asbestos (5–25 μg/cm 2) or H 2 O 2 (100–250 μm)) for 24 h. Overexpression of wild …
(Aco-2) each reduce oxidant-induced alveolar epithelial cell (AEC) apoptosis, but it is
unclear whether protection occurs by preventing AEC mitochondrial DNA (mtDNA) damage.
Using quantitative PCR-based measurements of mitochondrial and nuclear DNA damage,
mtDNA damage was preferentially noted in AEC after exposure to oxidative stress (eg
amosite asbestos (5–25 μg/cm 2) or H 2 O 2 (100–250 μm)) for 24 h. Overexpression of wild …