Neuropeptide FF-like immunoreactivity in human plasma

DM Sundblom, P Panula, F Fyhrquist - Peptides, 1995 - Elsevier
In order to examine whether neuropeptide FF (NPFF), an octapeptide with pain-modulating
and blood pressure-raising properties in the rat, is present in circulating human blood, a
radioimmunoassay (RIA) was established. Using this highly specific and sensitive RIA, the
mean concentration of NPFF in human plasma was 2.9 pg/ml±1.1 (n= 111). The
concentration did not correlate with age or sex. Reversed-phase high pressure liquid
chromatography (HPLC) followed by RIA using two different antisera for NPFF showed that …