Fibrinogen inhibits neurite outgrowth via β3 integrin-mediated phosphorylation of the EGF receptor

C Schachtrup, P Lu, LL Jones, JK Lee… - Proceedings of the …, 2007 - National Acad Sciences
C Schachtrup, P Lu, LL Jones, JK Lee, J Lu, BD Sachs, B Zheng, K Akassoglou
Proceedings of the National Academy of Sciences, 2007National Acad Sciences
Changes in the molecular and cellular composition of the CNS after injury or disease result
in the formation of an inhibitory environment that inhibits the regeneration of adult
mammalian CNS neurons. Although a dramatic change in the CNS environment after
traumatic injury or disease is hemorrhage because of vascular rupture or leakage of the
blood–brain barrier, the potential role for blood proteins in repair processes remains
unknown. Here we show that the blood protein fibrinogen is an inhibitor of neurite outgrowth …
Changes in the molecular and cellular composition of the CNS after injury or disease result in the formation of an inhibitory environment that inhibits the regeneration of adult mammalian CNS neurons. Although a dramatic change in the CNS environment after traumatic injury or disease is hemorrhage because of vascular rupture or leakage of the blood–brain barrier, the potential role for blood proteins in repair processes remains unknown. Here we show that the blood protein fibrinogen is an inhibitor of neurite outgrowth that is massively deposited in the spinal cord after injury. We show that fibrinogen acts as a ligand for β3 integrin and induces the transactivation of EGF receptor (EGFR) in neurons. Fibrinogen-mediated inhibition of neurite outgrowth is reversed by blocking either β3 integrin or phoshorylation of EGFR. Inhibition of Src family kinases that mediate the cross-talk between integrin and growth factor receptors rescue the fibrinogen-induced phosphorylation of EGFR. These results identify fibrinogen as the first blood-derived inhibitor of neurite outgrowth and suggest fibrinogen-induced EGFR transactivation on neuronal cells as a molecular link between vascular and neuronal damage in the CNS after injury.
National Acad Sciences