An OLTAM system for analysis of brown/beige fat thermogenic activity
DI Kim, J Liao, MP Emont, MJ Park, H Jun… - International Journal of …, 2018 - nature.com
International Journal of Obesity, 2018•nature.com
Methods: We detected increased luciferase activity upon thermogenic activation in
interscapular brown and inguinal subcutaneous fat in ODD-Luc mice, a hypoxia reporter
mouse model. We then developed an OLTAM (O DD-L uc based T hermogenic A ctivity M
easurement) system to assay thermogenic fat cell activity. Results: In both primary murine
and human adipocytes and an immortalized adipose cell line that were transduced with the
OLTAM system, luciferase activity can be readily measured and visualized by …
interscapular brown and inguinal subcutaneous fat in ODD-Luc mice, a hypoxia reporter
mouse model. We then developed an OLTAM (O DD-L uc based T hermogenic A ctivity M
easurement) system to assay thermogenic fat cell activity. Results: In both primary murine
and human adipocytes and an immortalized adipose cell line that were transduced with the
OLTAM system, luciferase activity can be readily measured and visualized by …
Methods:
We detected increased luciferase activity upon thermogenic activation in interscapular brown and inguinal subcutaneous fat in ODD-Luc mice, a hypoxia reporter mouse model. We then developed an OLTAM (O DD-L uc based T hermogenic A ctivity M easurement) system to assay thermogenic fat cell activity.
Results:
In both primary murine and human adipocytes and an immortalized adipose cell line that were transduced with the OLTAM system, luciferase activity can be readily measured and visualized by bioluminescence imaging in response to a variety of stimuli, including UCP1-independent thermogenic signaling. This system can offer a convenient method to assay thermogenic activity for both basic and translational research.
Conclusions:
The OLTAM system offers a convenient way to measure the activation of thermogenic fat and presents opportunities to discover novel signaling pathways and unknown compounds targeting metabolically active adipocytes to counteract human obesity.
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