Adenovirus-mediated Wild-type p53 Gene Transfer and Overexpression Induces Apoptosis of Human Glioma Cells Independent of Endogenous p53 Status
H Li, H Lochmüller, VW Yong, G Karpati… - … of Neuropathology & …, 1997 - academic.oup.com
H Li, H Lochmüller, VW Yong, G Karpati, J Nalbantoglu
Journal of Neuropathology & Experimental Neurology, 1997•academic.oup.comMutation or inactivation of the p53 tumor suppression gene is an early alteration in the
transformation of glial cells to gliomas. To study the effect of exogenous wild-type p53 on
glioma cell growth, human glioma lines U251 MG, U87 MG and A172 were infected with an
adenovirus vector expressing either wild-type p53 or bacterial lacZ. Rapid cell death
occurred only in the p53-transduced cell lines and was characterized by nuclear
condensation, formation of nucleosomal DNA ladders, and positive in situ end-labeling of …
transformation of glial cells to gliomas. To study the effect of exogenous wild-type p53 on
glioma cell growth, human glioma lines U251 MG, U87 MG and A172 were infected with an
adenovirus vector expressing either wild-type p53 or bacterial lacZ. Rapid cell death
occurred only in the p53-transduced cell lines and was characterized by nuclear
condensation, formation of nucleosomal DNA ladders, and positive in situ end-labeling of …
Abstract
Mutation or inactivation of the p53 tumor suppression gene is an early alteration in the transformation of glial cells to gliomas. To study the effect of exogenous wild-type p53 on glioma cell growth, human glioma lines U251 MG, U87 MG and A172 were infected with an adenovirus vector expressing either wild-type p53 or bacterial lacZ. Rapid cell death occurred only in the p53-transduced cell lines and was characterized by nuclear condensation, formation of nucleosomal DNA ladders, and positive in situ end-labeling of DNA, suggesting that apoptosis had been induced. The U87 MG cell line that contains wild-type p53 as evidenced by wild-type p53-dependent transcription activity also underwent apoptosis within 2 to 3 days after infection. These results suggest that the presence of endogenous wild-type p53 does not preclude apoptosis by overexpression of exogenous p53.
Oxford University Press