Unbudded G2 as well as Gj arrest in the stationary phase of the basidiomycetous yeast Cryptococcus neoformans
K Takeo, R Tanaka, M Miyaji… - FEMS microbiology …, 1995 - academic.oup.com
K Takeo, R Tanaka, M Miyaji, K Nishimura
FEMS microbiology letters, 1995•academic.oup.comStationary-phase cells of Cryptococcus neoformans displayed two morphological
characteristics: virtually all the cells were unbudded even in the early stationary phase and
even when grown in rich media, and average cell size increased from that of exponential-
phase cells. DNA contents for small and large stationary-phase cells were determined by
quantitative fluorescence microscopy after DNA staining with propidium iodide or DAPI.
Small cells contained G, DNA, whereas large unbudded cells had either a G2 or G1 DNA …
characteristics: virtually all the cells were unbudded even in the early stationary phase and
even when grown in rich media, and average cell size increased from that of exponential-
phase cells. DNA contents for small and large stationary-phase cells were determined by
quantitative fluorescence microscopy after DNA staining with propidium iodide or DAPI.
Small cells contained G, DNA, whereas large unbudded cells had either a G2 or G1 DNA …
Abstract
Stationary-phase cells of Cryptococcus neoformans displayed two morphological characteristics: virtually all the cells were unbudded even in the early stationary phase and even when grown in rich media, and average cell size increased from that of exponential-phase cells. DNA contents for small and large stationary-phase cells were determined by quantitative fluorescence microscopy after DNA staining with propidium iodide or DAPI. Small cells contained G, DNA, whereas large unbudded cells had either a G2 or G1 DNA content, indicating that Cr. neoformans can enter into the stationary phase from either the G1 or G2 period.
Oxford University Press