[HTML][HTML] Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay
YE Karapetyan, P Saá, SP Mahal, GF Sferrazza… - PLoS …, 2009 - journals.plos.org
YE Karapetyan, P Saá, SP Mahal, GF Sferrazza, A Sherman, N Salès, C Weissmann…
PLoS One, 2009•journals.plos.orgPrion strain identification has been hitherto achieved using time-consuming incubation time
determinations in one or more mouse lines and elaborate neuropathological assessment. In
the present work, we make a detailed study of the properties of PrP-overproducing Tg a 20
mice. We show that in these mice the four prion strains examined are rapidly and faithfully
amplified and can subsequently be discriminated by a cell-based procedure, the Cell Panel
Assay.
determinations in one or more mouse lines and elaborate neuropathological assessment. In
the present work, we make a detailed study of the properties of PrP-overproducing Tg a 20
mice. We show that in these mice the four prion strains examined are rapidly and faithfully
amplified and can subsequently be discriminated by a cell-based procedure, the Cell Panel
Assay.
Prion strain identification has been hitherto achieved using time-consuming incubation time determinations in one or more mouse lines and elaborate neuropathological assessment. In the present work, we make a detailed study of the properties of PrP-overproducing Tga20 mice. We show that in these mice the four prion strains examined are rapidly and faithfully amplified and can subsequently be discriminated by a cell-based procedure, the Cell Panel Assay.
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