Intracellular translocation of iodine-125-labeled insulin: direct demonstration in isolated hepatocytes
P Gorden, JL Carpentier, P Freychet, A LeCam, L Orci - Science, 1978 - science.org
P Gorden, JL Carpentier, P Freychet, A LeCam, L Orci
Science, 1978•science.orgInsulin labeled with iodine-125 binds to receptors on isolated rat hepatocytes. At low
temperatures initial binding is restricted to the plasma membrane as detected by direct
quantitative autoradiographic analysis with the electron microscope. With increasing time
and temperature of incubation there is a systematic and progressive translocation of
autoradiographic grains to a highly limited area of the cell periphery representing no more
than 15 percent of the radius of the cell.
temperatures initial binding is restricted to the plasma membrane as detected by direct
quantitative autoradiographic analysis with the electron microscope. With increasing time
and temperature of incubation there is a systematic and progressive translocation of
autoradiographic grains to a highly limited area of the cell periphery representing no more
than 15 percent of the radius of the cell.
Insulin labeled with iodine-125 binds to receptors on isolated rat hepatocytes. At low temperatures initial binding is restricted to the plasma membrane as detected by direct quantitative autoradiographic analysis with the electron microscope. With increasing time and temperature of incubation there is a systematic and progressive translocation of autoradiographic grains to a highly limited area of the cell periphery representing no more than 15 percent of the radius of the cell.
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