Chemical synthesis of genes for human insulin.
R Crea, A Kraszewski, T Hirose… - Proceedings of the …, 1978 - National Acad Sciences
R Crea, A Kraszewski, T Hirose, K Itakura
Proceedings of the National Academy of Sciences, 1978•National Acad SciencesA rapid chemical procedure has been developed and used for the synthesis of 29
oligodeoxyribonucleotides to build synthetic genes for human insulin. The gene for insulin B
chain, 104 base pairs, and the one for A chain, 77 base pairs, were designed from the amino
acid sequence of human polypeptides. They bear single-stranded cohesive termini for the
EcoRI and BamHI restriction endonucleases and are designed to be inserted separately into
a pBR322 plasmid. The synthetic fragments, deca-to pentadecanucleotides, were …
oligodeoxyribonucleotides to build synthetic genes for human insulin. The gene for insulin B
chain, 104 base pairs, and the one for A chain, 77 base pairs, were designed from the amino
acid sequence of human polypeptides. They bear single-stranded cohesive termini for the
EcoRI and BamHI restriction endonucleases and are designed to be inserted separately into
a pBR322 plasmid. The synthetic fragments, deca-to pentadecanucleotides, were …
A rapid chemical procedure has been developed and used for the synthesis of 29 oligodeoxyribonucleotides to build synthetic genes for human insulin. The gene for insulin B chain, 104 base pairs, and the one for A chain, 77 base pairs, were designed from the amino acid sequence of human polypeptides. They bear single-stranded cohesive termini for the EcoRI and BamHI restriction endonucleases and are designed to be inserted separately into a pBR322 plasmid. The synthetic fragments, deca- to pentadecanucleotides, were synthesized by a block phosphotriester method with trinucleotides as building blocks. Final purification was by high-performance liquid chromatography. All 29 oligonucleotides were pure and had the correct sequences.
National Acad Sciences
