[HTML][HTML] Generation of iPSC line from desmin-related cardiomyopathy patient carrying splice site mutation of DES gene
A Khudiakov, D Kostina, A Zlotina, T Nikulina… - Stem cell …, 2017 - Elsevier
Stem cell research, 2017•Elsevier
Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal
multipotent stromal cells carrying desmin (DES) gene heterozygous splice site mutation
using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2,
CMYC were delivered using Sendai viruses. iPSCs were characterized by sequencing,
karyotype analysis, STR analysis, immunocytochemistry, RT-PCR and teratoma formation.
multipotent stromal cells carrying desmin (DES) gene heterozygous splice site mutation
using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2,
CMYC were delivered using Sendai viruses. iPSCs were characterized by sequencing,
karyotype analysis, STR analysis, immunocytochemistry, RT-PCR and teratoma formation.
Abstract
Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying desmin (DES) gene heterozygous splice site mutation using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. iPSCs were characterized by sequencing, karyotype analysis, STR analysis, immunocytochemistry, RT-PCR and teratoma formation.
Elsevier