[HTML][HTML] Increased apolipoprotein-B: A1 ratio predicts cardiometabolic risk in patients with juvenile onset SLE
GA Robinson, KE Waddington, L Coelewij, J Peng… - …, 2021 - thelancet.com
EBioMedicine, 2021•thelancet.com
Background Cardiovascular disease is a leading cause of mortality in patients with juvenile-
onset systemic lupus erythematosus (JSLE). Traditional factors for cardiovascular risk (CVR)
prediction are less robust in younger patients. More reliable CVR biomarkers are needed for
JSLE patient stratification and to identify therapeutic approaches to reduce cardiovascular
morbidity and mortality in JSLE. Methods Serum metabolomic analysis (including> 200
lipoprotein measures) was performed on a discovery (n= 31, median age 19) and validation …
onset systemic lupus erythematosus (JSLE). Traditional factors for cardiovascular risk (CVR)
prediction are less robust in younger patients. More reliable CVR biomarkers are needed for
JSLE patient stratification and to identify therapeutic approaches to reduce cardiovascular
morbidity and mortality in JSLE. Methods Serum metabolomic analysis (including> 200
lipoprotein measures) was performed on a discovery (n= 31, median age 19) and validation …
Background
Cardiovascular disease is a leading cause of mortality in patients with juvenile-onset systemic lupus erythematosus (JSLE). Traditional factors for cardiovascular risk (CVR) prediction are less robust in younger patients. More reliable CVR biomarkers are needed for JSLE patient stratification and to identify therapeutic approaches to reduce cardiovascular morbidity and mortality in JSLE.
Methods
Serum metabolomic analysis (including >200 lipoprotein measures) was performed on a discovery (n=31, median age 19) and validation (n=31, median age 19) cohort of JSLE patients. Data was analysed using cluster, receiver operating characteristic analysis and logistic regression. RNA-sequencing assessed gene expression in matched patient samples.
Findings
Hierarchical clustering of lipoprotein measures identified and validated two unique JSLE groups. Group-1 had an atherogenic and Group-2 had an atheroprotective lipoprotien profile. Apolipoprotein(Apo)B:ApoA1 distinguished the two groups with high specificity (96.2%) and sensitivity (96.7%). JSLE patients with high ApoB:ApoA1 ratio had increased CD8+ T-cell frequencies and a CD8+ T-cell transcriptomic profile enriched in genes associated with atherogenic processes including interferon signaling. These metabolic and immune signatures overlapped statistically significantly with lipid biomarkers associated with sub-clinical atherosclerosis in adult SLE patients and with genes overexpressed in T-cells from human atherosclerotic plaque respectively. Finally, baseline ApoB:ApoA1 ratio correlated positively with SLE disease activity index (r=0.43, p=0.0009) and negatively with Lupus Low Disease Activity State (r=-0.43, p=0.0009) over 5-year follow-up.
Interpretation
Multi-omic analysis identified high ApoB:ApoA1 as a potential biomarker of increased cardiometabolic risk and worse clinical outcomes in JSLE. ApoB:ApoA1 could help identify patients that require increased disease monitoring, lipid modification or lifestyle changes.
Funding
Lupus UK, The Rosetrees Trust, British Heart Foundation, UCL & Birkbeck MRC Doctoral Training Programme and Versus Arthritis.
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