[HTML][HTML] Phenotypic correction of primary Fanconi anemia T cells with retroviral vectors as a diagnostic tool
H Hanenberg, SD Batish, KE Pollok, L Vieten… - Experimental …, 2002 - Elsevier
OBJECTIVE: The aim of this study was to develop a rapid laboratory procedure that is
capable of subtyping Fanconi anemia (FA) complementation groups FA-A, FA-C, FA-G, and
FA-nonACG patients from a small amount of peripheral blood. MATERIALS AND METHODS:
For this test, primary peripheral blood-derived FA T cells were transduced with oncoretroviral
vectors that expressed FANCA, FANCC, or FANCG cDNA. We achieved a high efficiency of
gene transfer into primary FA T cells by using the fibronectin fragment CH296 during …
capable of subtyping Fanconi anemia (FA) complementation groups FA-A, FA-C, FA-G, and
FA-nonACG patients from a small amount of peripheral blood. MATERIALS AND METHODS:
For this test, primary peripheral blood-derived FA T cells were transduced with oncoretroviral
vectors that expressed FANCA, FANCC, or FANCG cDNA. We achieved a high efficiency of
gene transfer into primary FA T cells by using the fibronectin fragment CH296 during …