The C type lectins DC-SIGN and L-SIGN: receptors for viral glycoproteins
Abstract DC-SIGN and L-SIGN are C-type lectins that recognize carbohydrate structures
present on viral glycoproteins and function as attachment factors for several enveloped
viruses. DC-SIGN and L-SIGN enhance viral entry and facilitate infection of cells that
express the cognate entry receptor (cis-infection). They are also able to capture viruses and
transfer viral infections to other target cells (trans-infection). In this chapter, we will give an
overview of protocols used to produce soluble viral glycoproteins at high levels and to study …
present on viral glycoproteins and function as attachment factors for several enveloped
viruses. DC-SIGN and L-SIGN enhance viral entry and facilitate infection of cells that
express the cognate entry receptor (cis-infection). They are also able to capture viruses and
transfer viral infections to other target cells (trans-infection). In this chapter, we will give an
overview of protocols used to produce soluble viral glycoproteins at high levels and to study …
Abstract
DC-SIGN and L-SIGN are C-type lectins that recognize carbohydrate structures present on viral glycoproteins and function as attachment factors for several enveloped viruses. DC-SIGN and L-SIGN enhance viral entry and facilitate infection of cells that express the cognate entry receptor (cis-infection). They are also able to capture viruses and transfer viral infections to other target cells (trans-infection). In this chapter, we will give an overview of protocols used to produce soluble viral glycoproteins at high levels and to study the molecular basis of viruses/DC-SIGN and L-SIGN binding and internalization. We will also describe techniques to investigate the molecular mechanisms by which DC-SIGN or L-SIGN spread viral infections.
Springer