Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes
JG Rheinwald, H Green - Nature, 1977 - nature.com
JG Rheinwald, H Green
Nature, 1977•nature.comThe culture lifetime of epidermal cells of newborn humans is increased from 50 to 150
generations by adding to the medium epidermal growth factor, a polypeptide mitogen. EGF
seems to delay senescence of the cells by maintaining them in a state further removed from
terminal differentiation. This effect is revealed by a greater ability of the cells to survive
subculture and initiate new colonies, but not necessarily by an increased growth rate.
generations by adding to the medium epidermal growth factor, a polypeptide mitogen. EGF
seems to delay senescence of the cells by maintaining them in a state further removed from
terminal differentiation. This effect is revealed by a greater ability of the cells to survive
subculture and initiate new colonies, but not necessarily by an increased growth rate.
Abstract
The culture lifetime of epidermal cells of newborn humans is increased from 50 to 150 generations by adding to the medium epidermal growth factor, a polypeptide mitogen. EGF seems to delay senescence of the cells by maintaining them in a state further removed from terminal differentiation. This effect is revealed by a greater ability of the cells to survive subculture and initiate new colonies, but not necessarily by an increased growth rate.
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