Modulation of Keratin Expression in Type 11 Pneumocytes by the Extracellular Matrix1-3
J Woodcock-Mitchell, SR Rannels, J Mitchell… - 1989 - atsjournals.org
J Woodcock-Mitchell, SR Rannels, J Mitchell, DE Rannels, RB Low
1989•atsjournals.orgThe expression of specific keratin Intermediate filaments during differentiation of rat type 11
pneumocytes in primary culture on various matrices was Investigated. Changes In keratin
expression were assessed using a monoclonal antlkeratln antibody, 24A3, known to react
strongly with alveolar epithelial cells In Injured lung. lYpe 11 cell differentiation was
modulated by culture on extracellular matrices known to either accelerate or retard loss of
differentiated morphology and metabolic function. During culture on a plastic or f1bronectln …
pneumocytes in primary culture on various matrices was Investigated. Changes In keratin
expression were assessed using a monoclonal antlkeratln antibody, 24A3, known to react
strongly with alveolar epithelial cells In Injured lung. lYpe 11 cell differentiation was
modulated by culture on extracellular matrices known to either accelerate or retard loss of
differentiated morphology and metabolic function. During culture on a plastic or f1bronectln …
Summary
The expression of specific keratin Intermediate filaments during differentiation of rat type 11 pneumocytes in primary culture on various matrices was Investigated. Changes In keratin expression were assessed using a monoclonal antlkeratln antibody, 24A3, known to react strongly with alveolar epithelial cells In Injured lung. lYpe 11 cell differentiation was modulated by culture on extracellular matrices known to either accelerate or retard loss of differentiated morphology and metabolic function. During culture on a plastic or f1bronectln-rlch surface, 1088 of cell differentiation correlates with Increased staining with 24A3 anti keratin antibody by Indirect Immunofluorescence and with increased abundance of a family of acidic 46, OOD-dalton keratin Isoforms detected
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