Western blot analysis of epimorphin/syntaxin 2 expression in transfected MIC 216 cell lines. Total cellular proteins (25 μg or 50 μg per lane as indicated) were separated by reduced SDS-PAGE on 4–12% acrylamide gels, transferred to nitrocellulose, incubated with the polyclonal epimorphin/syntaxin 2 antibody, and then visualized with enhanced chemiluminescence reagent. (a) The 34-kDa band corresponds to the epimorphin/syntaxin 2 protein. MIC C: native MIC 216 cell line grown in media without G418. pCEN V: MIC 216 cells transfected with empty pCEN vector. (b) EpiAS1 and EpiAS2: Two independent clonal pCEN-antisense epimorphin/syntaxin 2 cell lines. (c) EpiS1, EpiS2, EpiS3, and EpiS5: Four independent clonal pCEN-epimorphin/syntaxin 2 (sense) transfected cell lines. (d) Quantitation of epimorphin/syntaxin 2 expression by NIH Image analysis of specific bands. C, control; V, vector; S, sense; AS, antisense.